PENG Yan-li, et al. Research of UVA in affecting Cx43 protein among rabbit lens epithelia cells[J]. Journal of Chongqing Medical University, 2009,34(7):899-902.
PENG Yan-li, et al. Research of UVA in affecting Cx43 protein among rabbit lens epithelia cells[J]. Journal of Chongqing Medical University, 2009,34(7):899-902.DOI：
其Cx43蛋白的表达逐渐降低;并经近紫外线照射后正常定位于晶状体上皮细胞膜上的Cx43发生了内移。结论:证实近紫外线抑制晶状体上皮细胞通讯功能的机制可能与细胞缝隙连接通讯（Gap junction-mediated intercellular communication
GJIC）的关键蛋白Cx43蛋白表达的下降和功能定位的改变有关。
Abstract
Objective:To probe the mechanism of inhibition in gap junction-mediated intercellular communication (GJIC) of rabbit lens epithelia cells(RLECs) irradiated by UVA. Methods:RLECs of 2～3 generation were divided into normal control group and ultraviolet irradiation group (The UV intensity was 0.2 mW/cm2 under a UV lamp with fixed position 50 cm
RLECs were exposed to ultraviolet lamp at different times: 5
10
15 minutes). Western blot techniques were employed to detect the protein levels of Cx43 irradiated by UVA. By indirect immunofluorescence histochemistry we analyzed the localization of Cx43 in the RLECs. Results:In UVA irradiation group and normal control group
there was expression of Cx43. With the increasing intensity
the expression of Cx43 was decreasing. The immunnostaining of Cx43 in RLECs revealed that the internalization of Cx43 from membrance to plasma and nuclear was found in UVA irradiated RLECs. Conclusion: Cell molecule level study proved UVA irradiation can reduce GJIC of RLECs
which wis associated with perturbations in localization and number of Cx43 among RLECs.
关键词
近紫外线晶状体上皮细胞缝隙连接细胞通讯Cx43蛋白蛋白印迹法
Keywords
Ultraviolet A(UVA)Lens epithelia cellGap junction-mediated intercellular communicationConnexins43Western Blot
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