Objective:To investigate the effects of valproate sodium on P-CREB1 after hippocampal neuronal epileptiform discharge in rat.Methods:The neonate wistar rats were decapitated quickly to obtain the hippocampal neuron

Which were cultured in vitro

After the epileptiform discharge model of neuron was established

neurons were divided into control group

model group

low valproate dose group(50mg/L) and high dose group(100mg/L).Expression positions of P-CREB1 after epileptiform discharge were examined by immunofluorescence technique

and Western blot was used to examine the expression intensity of P-CREB1 in different group.Results:Through immunofluorescence

P-CREB1 was observed in the nucleus of each group

and the most intensive expression was found in model group.Through western blot

the expression tendency was found to be the same as the former Results

Moreover

after added valproate sodium

the expression of P-CREB1 decreased

and there was statistical significance of the difference between low valproate dose and high dose(P<0.05).Conclusion:P-CREB1 could be persistently activated in the hippocampal neuron

which presents epileptiform discharge.Valproate sodium could decrease the phosphorylation of P-CREB1 in effective dose.