Objective:To investigate the influences of different centrifugal conditions on the ultrastructure of cultured cells in the digestion centrifugation to find out the best speed and time of centrifugation.Methods:The cultured cells after digested

were divided into groups according to different digestion centrifuge speed and time

and the changes of cell morphology in each group at different centrifugation time and speed were compared under transmission electron microscope.Results:Enough cells could mot be collected with the centrifuge speed less than 800 r/min

8 min.The cells were deformed due to extrusion or the cytoplasm was torn with the speed of 2 000 r/min

15 min.With the speed of 800～1 500 r/min

8～15 min

it was more proper to prepare samples and preserve cell ultrastructure.Conclusion:It is ideal to prepare TEM samples of cultured cells by using the cells being digested and centrifuged with speed at 800 ~1 500 r/min and time of 8~15 minutes.