Objective:To improve the surgical technique to establish the arterialized orthotopic liver transplantion in rat.Methods:On the basis of two-cuff technique of Kamada

we improved the techniques of separaring

perfusing donor liver

the anastomosis of the suprahepatic inferior vena cava

and so on.And used the modified "stent" method to reestablish the hepatic artery.Results:80 cases of operation were completed.The time of donor operation

recipient operation and anhepatic time were(40±3) min

(50±5)min

and(16±2)min

respectively.The time of reestablishing suprahepatic inferior vena cava and hepatic artery were(6~9) min

(2~3) min

respectively.The survival rate at 48 h

1 week and 1 month were 96.3%

93.7%

and 87.5%

respectively.Conclusion:The modified method can enhance the stability and survival rate

which is an ideal method for establishing the arterialized orthotopic liver transplantation model in rat.