Objective:To investigate the effect of transfection with wild hepaCAM gene on biological behavior of the transitional cell carcinoma of bladder in vitro.Methods:Human TCCB cell line T24 was transfected with pEGFP-N2-hepaCAM

mock plasmid pEGFP-N2 with lipofectamine.Laser confocal microscopy was used to detect the location of hepaCAM in cells.Western bolt was used to determine the expression of target of hepaCAM in stable cells.The cell adhesive and motility ability were tested by cell spreading assay and matrigel invasion assay.Cell proliferation ability was investigated by MTT assay。Results:In well spread cells

hepaCAM localized on the perinuclear membrane

whereas in the cells which contacts

hepaCAM predominantly accumulated at the sites of cell-cell contacts on cell membrane.Cell clone with stable expression of hepaCAM

was acquired.In vitro experiments as cell spreading assays and matrigel invasion assays showed the cell adhesion and cell motility properties of hepaCAM group were apparently enhanced compared with the non-transfection or mock-vehicle groups.The MTT assay showed cell proliferation ability in the hepaCAM group was notably decreased when compared with the non-transfection or mock-vehicle groups.Conclusion:The hepaCAM gene can restrain malignant phenotypes of the human TCCB cells in vitro

and may inhibit the TCCB invasion and metastasis by influencing the function of some adherence and proliferation factors.[