Objective:To evaluate the expression of NF-κB and IL-6 and its relationship with liver regenerationafter 90% portal branch ligation in rats.Methods:Ninety-six SD rats were randomly divided into Sham-PBL group and portal vein branches ligation group.The weight of both ligated and unligated lobes of liver were measured at POD 0.5

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21 and 28.The morphological changes of the non-ligated liver lobes were observed by microscope.The expression of PCNA

NF-κB and IL-6 of the non-ligated liver lobes were studied with immunohistochemistry.The results were analyzed by medical statistics methods.Results:①Hepatic lobe at the ligated side diminished progressively after 90% portal branch ligation.The ratio of non-ligated lobes weight to the whole liver increased at POD1-5 period

and got "the plateau stage"at POD7;②PCNA index were increased at POD 0.5-7(P<0.05)

then gradually return to normal lately;③The expression of NF-κB and IL-6 of the non-ligated liver lobes were increased at POD0.5-5

then gradually return to normal in POD7-28;④The PCNA and NF-κB in liver had a positive correlation at POD0.5

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28.The expressions of PCNA and IL-6 had a positive correlation at POD1

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21.The expressions of NF-κB and IL-6 had a positive correlation at POD1

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28.Conclusion:The expression of NF-κB and IL-6 may has played a pivotal role in liver regeneration after 90% portal branch ligation in rats.