Objective:To evaluate the expression of 8-hydroxyguanine(8-OHdG) in type 2 diabetic rats and the effect of Probucol on it.Methods:25 rats were divided into 3 groups of normal rats(C)

diabetic rats(D)

and diabetic rats treated with Probucol(N).Content of malonaldehyde(MDA) and activity of antioxidant enzymes including superoxid edismutase(SOD) and 8-OHdG in the renal tissue were compared among the groups.In addition

blood glucose

serum creatinine(Cr) and blood ureanitrogen(BUN)were mesured;the renal tissue were observed by light micros copy and electron microscopy.Results:Compared with normal group

the level of BUN

Cr

MDA and 8-OHdG in Probucol untreated group were significantly increased

but the activity of SOD were decreased notably.In addition

the renal pathologic changes in Probucol untreated group was also decreased

but improved in the treared group obviously.Conclusion:Oxidative stress takes key point in the development of diabetic nephropathy.The expression of 8-OHdG in type2 diabetic rats were increased.Andantioxidation may be the possible mechanism of the nephroprotective action of Probucol.