Objective:To observe the protective effects of autologous bone marrow mesenchymal stem cells(BM-MSCs) on renal cell proliferation apoptosis during cold ischemia-reperfusion injury(IRI) in rabbit and to probe the mechanism of protective action on kidney. Methods:45 rabbits were divided randomly into three groups(n=15): Sham operation group( group Ⅰ)

control group of IRI( group Ⅱ and BM-MSCs transplantation group(group Ⅲ). After isolating the BM-MSCs of rabbits in group Ⅲ

to establish the rabbit models of renal IRI. Autologous BM-MSCs were infuded into rabbit in group Ⅲ

and the equivalent physiological saline in rabbit in group Ⅰ and Ⅱ. The detection was taken at the timepoints of 3

7 and 14 days after renal ischemia-reperfusion respectively. The serum levels of Crea and Urea were detected to evaluate the renal function

and Bax

Bcl-2 and PCNA in kidney were dectected with immunohistochemical staining. The apoptosis of cells was detected with TUNEL method. Results:The serum levels of Crea and Urea in group Ⅲ at different timepoint were lower than those in group Ⅱ significantly(P<0.05). The expressions of Bcl-2 protein and PI of renal tubular epithelia cells in group Ⅲat different timepoint were more than those in group Ⅱsignificantly(P<0.05)and the expressions of Bax protein and AI of renal tubular epithelia cells in group Ⅲless than those in group Ⅱ significantly(P<0.05). Conclusions:BM-MSCs can promote cell proliferation and suppress apoptosis in kindey through up-regulating the expression of Bcl-2 protein and down-regulating the expression of Bax protein after renal IRI

thereby

decrease the serum levels of Crea and Urea

and promote the renal functional repair.