Objective:To observe therapeutic effects of plasmid pcDNA3.1/hFasL on mice with Graves’ disease.Methods:Three groups were set in this study.Animals in the control group(10 mice) were immunized with splenocytes sensitized by blank plasmid pcDNA3.1(+)

and then treated with pcDNA3.1(+).Animals in the model group(19 mice) and treated group(19 mice) were all immunized with splenocytes sensitized by hTSHR

then the latter were injected with pcDNA3.1(+)/hFasL.Results:47.4%of thyroid follicular epithelia in the model group displayed hypertrophy and hyperplasia

and the follicles cavity contained little colloid.Compared with the control group

elevated TT4 and TRAb and reduced TSH levels(P<0.05) were also observed in the model group.In the treated group

only 15.8% of thyroid follicular epithelia showed pathologic changes of GD with heterochromatin margination.There was no statistical significance in the levels of TT4

TRAb

and TSH between the treated group and the control group.Conclusion:Gene therapy by administration of plasmid pcDNA3.1/hFasL has made a curative therapeutic effects on Graves’ disease in animal model.