Objective:To study the effect of survivin expression on the mature process of dendritic cells from mouse bone marrow. Methods:The cells in marrow as the precurosor of the dendritic cells were taken from mouse in bioclean condition. Then the iDC were induced by the precurosor of DCs with GM-CSF and IL-4 in medium. ThemDC were induced by the precurosor of DCs with GM-CSF

IL-4

and TNF-α in medium. Survivin was compared by RT-PCR

and Western-bolt among these three stages. Results:The RT-PCR showed that the expression ratios of survivin mRNA among the precurosor of DCs

iDC

and mDC were(0.735±0.081)

(0.596±0.073

P<0.05)

and(0.393±0.052

P<0.01). Respectively

the Westernblot showed that the expression ratios of survivin proteinum among the above-mentioned DC’s three stages were(0.767±0.045)

(0.396±0.016

P<0.05)

and(0.168±0.009

P<0.01). Conclusion: Compared with thesurvivin in iDC and mDC

survivin in the precurosor of DCs was expressed more highly;And it was more highly expressed in iDC than in mDC. This conclusion is providing fundamental basis for our follow-up experiments.