Objective:To construct double fusogenic oncolytic virus and evaluate its antitumor activity against A549.Methods:The double fusogenic oncolytic virus was incorporated with both fusion mechanisms into a single oncolytic HSV throug insertion of the hyperfusogenic glycoprotein gene of gibbon ape leukemia virus(GALV.fus) into HSV-1.Syncytial phenotype was observed with microscope.Double fusogenic virus antitumor activity against A549

compared with simple oncolytic HSV

was evaluated in vitro and vivo.Results:The A549 cell infected with the two kind of oncolytic virus showed syncytial phenotype respectively.A distinctive syncytial phenotype was observed with the double fusogenic oncolytic HSV(Synco-2D) under microscope.In vitro characterization of Synco-2D showed that this virus produced a distinctive antitumor ability

compared with that of a nonfusogenic virus.When oncolytic virus was injected directly into the abdominal cavity of mice bearing A549 cells

Synco-2D eradicated tumor masses significantly as compared with single oncolytic HSV.Conclusion:A double fusogenic oncolytic HSV can significantly increase killing ability against lung adenoca rcinoma cell A549.