Objective:To observe the influence of the RhEGF on the recruiting and settling of the MSCs on injured part of the esophageal mucosa.Methods:The SD rats were investigated and divided into groups randomly.SD rat MSCs marked by DAPI were transplanted into the experimental groups and control groups rats by tail vein injection and local injection respectively.The experimental groups were given rhEGF.After 2 weeks

the esophageal tissues were taken to observe the recruitment and settlement of implanted cells with the laser scanning microscope.Results: Locally injected group

the group with esophageal injury and given rhEGF could be seen to be with more DAPI labeled cells;the group without rhEGF could be seen to be with DAPI less labeled cells.There was significant difference between these two groups(P<0.05).There was significant difference between the normal group and locally injected group(P<0.05)

both in rhEGF given rats and rats without rhEGF.Tail vein graft group:There were few DAPI labeled cells observed.There was significant difference between the tail vein graft group with rhEGF and normal group(P<0.05)

and there was no significant difference between the tail vein graft but without rhEGF group and normal group(P>0.05).Conclusion: MSCs can be recruited and settled in rat damage esophagus tissue.There are different results between tail vein and local injection.rhEGF can influence the recruitment and settlement of MSCs in rat injury esophagus.