Objective:To investigate the effects of exogenous hydrogen sulfide(H2S) on the production of interleukin 6(IL-6)

interleukin 8(IL-8)

and nitric oxide(NO) in keratinocytes induced by tumor necrosis factor alpha(TNF-α)

and to explore whether nuclear factor kappa B(NF-κB) signaling pathway is involved in this process.Methods:HaCat cells were induced by TNF-α to secrete inflammatory factors

and the H2S donor

sodium hydrosulfide(NaHS)

was added to the medium with different concentrations.The levels of IL-6 and IL-8 secreted by HaCat cells were measured by ELISA method;RT-PCR was performed to detect IL-6 and IL-8 mRNA levels.Griess method was used to determine the production of NO.The intracellular inducible nitric oxide synthase(iNOS)

phosphorylation of I-kappa B-alpha(p-IKB-α) and nucleus NF-κB P65 levels were assayed by Western blot.Results:10 ng/ml TNF-α could significantly increase the transcriptional and secretion levels of IL-6 and IL-8 in HaCat cells.The NO production in the culture supernatant and the intracellular level of iNOS were significantly increased;intracellular p-IKB-α and nucleus P65 levels were also significantly increased.20~400 μmol/L NaHS was given as pretreatment one hour prior to the induction by TNF-α.The results showed dose-dependent inhibition of the secretion of IL-6

IL-8 and NO

and partial inhibition of NF-κB signaling pathway.Conclusion:H2S can inhibit TNF-α-induced inflammatory response of keratinocytes via NF-κB pathway.