Objective:To investigate the influence of silencing clusterin(CLU) by RNA interference on radiosensitivity of human ovarian carcinoma SKOV3 cell-line.Methods:The expression of CLU gene was silenced by siRNA against it

Western blot was performed to detect the expression of CLU on protein level.After receiving different doses of radiation(2

4

6 Gy)

The cell survival rate was detected by MTT assay

and the cloning efficiency was also evaluated by the assay

the apoptotic rate was detected by flow cytometry.Results:The siRNA target clu specifically down regulated the expression of CLU significantly.The MTT and colony forming results showed that the cell survival and cloning efficiency became significantly lower with the increasing dose of ionizing irradiation

the capacity of proliferation and cloning efficiency was inhibited obviously(P<0.05).The apoptotic rate became significantly higher with the increasing dose of ionizing irradiation

which reached(24.32±1.75)% after 2 Gy(P<0.05)

and increased about 12% compared with control group.Conclusion:The CLU-silencing can significantly enhance the radio-sensitivity of ovarian carcinoma SKOV3 cell-line in vitro

and the CLU gene may be a promising target for basic research of ovarian carcinoma.