Objective:To investigate the effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell.Methods:Marrow precursor cells were cultured in RPMI1640 medium containing rmGM-CSF and rmIL-4 +either cs-Tca8113 or not.Control groups were cultured without any cs-Tca8113.The morphology of DCs were observed under optical microscope;the number of survive cells was calculated by trypan blue;the phenotypes of cells were analyzed by flow cytometry.All results were analyzed statistically by LSD-t.Results:The cells cultured in tumor microenvironment appeared typical morphotype and phenotype.Microenvironment of low dose cs-Tca8113 stimulated DCs to mature.Microenvironment of medium dose cs-Tca8113 promoted bone marrow precursor to differentiate into imDC.Microenvironment of high dose cs-Tca8113 inhibited DCs to differentiate and mature.Conclusion:Bone marrow precursor cells can be successfully induced into DCs with typical characteristics in the microenvironment of Tca8113.The multiple effects of Tca8113 microenvironment on the growth and differentiation of DCs are closely related to the concentration of cs-Tca8113.