Objective:To assess the value of high frequency ultrasound in monitoring the growth of xenograft neuroblastoma in immunocompetent sprague dawley(SD) mouse model.Methods:Fifty-three SD mice were implanted with human neuroblastoma(SK-N-SH) cell suspension.All of the mice were injected retroperitoneally in adrenal area.Five mice were chosen randomly and the size

internal echo and blood supply of tumor were observed by high frequency ultrasound at 5 d～6 weeks after the first injection.After the examination

the mice were dissected under narcotics and the tumors were dissociated immediately.The transversal and sagittal diameter was measured by high frequency ultrasound and straightedge.Tumors were fixed for HE staining.Results:The SD mouse model of xenograft neuroblastoma was made successfully

and the success rate was 100%.The volume of tumor measured by high frequency ultrasound and straightedge had strong correlation(r=0.993

P<0.001).High frequency ultrasound could also observe blood supply and necrosis of the tumors at real-time.The fast period of tumor growth with the richest blood supply appeared 5～13 days after injection.Conclusion:High frequency ultrasound provides an accurate

practical method to monitor the growth of xenograft neuroblastoma in SD mouse model.