Objective:To investigate the mechanism of TLR4/NF-κB on Hcy to promote the damage of the vein endothelial cells on plaques of atherosclerosis.Methods:Cultured HUVECs were treated by 0

50

100

200 μmol/L and 500 μmol/L Hcy

100 μmol/L Hcy+30 μmol/L(VitaminB12+Folic acid) concentrations of Hcy for 72 h.The activity of HUVECs was examined by MTT assay.The MDA and NF-κB contents and SOD activity were measured by chromatometry and ELISA.The mRNA and protein expressions of TLR4 were detected by fluorescent quantitation PCR and Western blot.Results:MTT test showed that Hcy could restrain the activity of HUVECs after being treated by Hcy for 72 h.The activity of 100 to 500 μmol/L Hcy compared to control cells were significant(P<0.05)

but other cells compared to control cells were not significant(P>0.05);the increasing concentration of Hcy increased MDA and NF-κB contents as well as the mRNA and protein expressions of TLR4

but it reduced the SOD contents.The activity of 100

200 μmol/L and 500 μmol/L Hcy compared to control cells was significant(P<0.05)

but other cells compared to control cells were not significant(P>0.05).Conclusion:The TLR4/NF-κB may act as one of important mechanisms in Hcy to promote the damage of HUVECs

in which MDA and SOD may play an important role.