Objective：To evaluate the reliability and validity of questionnaire of health education needs during the period of chemotherapy in patients with gynecological cancer. Methods：The draft questionnaire was developed on the basis of domestic and in－ternational literature review，semi-structured interviews and 3 rounds of experts’ consultation. Then，a questionnaire survey with this draft questionnaire was conducted among one hundred and seventy-three patients with gynecological cancer in the First Affiliated Hospital of Chongqing Medical University from November 2016 to January 2017. The reliability of the questionnaire was tested by in－ternal consistency reliability and test-retest reliability. Meanwhile，the validity of the questionnaire was evaluated by content validity and construct validity. Results：Nineteen items of the questionnaire in six domains were eventually formed，including cognitive health education needs，physical health education needs，safety health education needs，attribution health education needs，self-esteem health education needs and self-realization health education needs. Exploratory factor analysis extracted six common factor totally，the cu－mulative variance contribution rate is 60.77%. The total Cronbach’s α coefficient of the questionnaire was 0.817，and the Cronbach’s αcoefficient of each domain was between 0.649 and 0.764. Test-retest reliability was 0.829，and the content validity index was 0.85. Conclusion：The developed questionnaire has a good reliability and validity. It can be used to evaluate the level of health education needs for gynecological cancer patients during chemotherapy period，and provide reference for medical staff to implement health edu－cation for them.