Objective：To explore the application effect of JUC to prepare skin in gynecological laparotomy surgery. Methods：Patients of 198 in The First Affiliated Hospital of Chongqing Medical University from October 2015 to October 2016 were selected，who aged between 20 and 70 years old and were underwent gynecological laparotomy surgery to treatment gynecological benign disease and had surgical parts in the lower abdomen，and were randomly divided into the experimental group of 100 cases and the control group of 98 cases. JUC was used to prepared skin in the experimental group，while the iodophor lubricant was used in the control group. After preparing skin，bacteriological testing，skin preparing time，skin reaction and postoperative incision healing were immediately com－pared. Results：Results of bacteriological testing，skin preparing time and skin reaction of two groups had no significant difference（P>0.05），but its postoperative incision healing had significant difference（P<0.05）. Conclusion：Using JUC to prepare skin can reduce the rate of incision infection after gynecological laparotomy sur-gery and enhance the postoperative incision healing rate. In ad－dition，its operation method is simple and worthy of promotion.