Objective：To study the effect of restraint stress on the inflammation changes of atopic dermatitis animal model. Methods： Twenty eight 6 to 8 weeks’ female BALB/c mice were divided into 4 groups：DNCB-induced model restraint stress group，DNCB-in－duced model restraint stress control group，acetone-induced model restraint stress group and acetone-induced model restraint stress control group. At the 14th day，mice were sacrificed and dermal infiltrated inflammatory cells and mRNA levels of inflammatory cy－tokines of skin lesions were analyzed by microscope histopathology and Q-PCR respectively. Results：In DNCB-induced model re－straint stress control group，the numbers of infiltrated inflammatory cells in dermal were significantly lower than DNCB-induced model restraint stress group（42.710±3.944） vs. （26.000±2.410）（P<0.01）. The mRNA levels of IL-4 and IL-13 were significantly elevated in DNCB-induced model restraint stress group. Conclusion：Restraint stress might accelerate the inflammatory response of atopic der－matitis.