LHRHa靶向紫杉醇脂质体的制备及体外抗肿瘤作用

陈佳; 孙江川; 常淑芳; 朱轶; 朱深银

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LHRHa靶向紫杉醇脂质体的制备及体外抗肿瘤作用

2024神经精神疾病的治疗 | 更新时间：2023-12-18

- LHRHa靶向紫杉醇脂质体的制备及体外抗肿瘤作用
- Preparation of LHRHa Paclitaxel liposomes and its anti tumor effect in vitro
- 重庆医科大学学报 2012年37卷第4期页码：298-301
- 作者机构：
  
  1. 重庆医科大学附属第二医院妇产科,重庆,400010
  2. 重庆医科大学附属第一医院药剂科,重庆,400016
- 作者简介：
- 基金信息：
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- 纸质出版日期：2012，
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陈佳, 孙江川, 常淑芳, 等. LHRHa靶向紫杉醇脂质体的制备及体外抗肿瘤作用[J]. 重庆医科大学学报, 2012,37(4):298-301.

CHEN Jia, SUN Jiangchuan, CHANG Shufang, et al. Preparation of LHRHa Paclitaxel liposomes and its anti tumor effect in vitro[J]. Journal of Chongqing Medical University, 2012,37(4):298-301.
陈佳, 孙江川, 常淑芳, 等. LHRHa靶向紫杉醇脂质体的制备及体外抗肿瘤作用[J]. 重庆医科大学学报, 2012,37(4):298-301. DOI：

CHEN Jia, SUN Jiangchuan, CHANG Shufang, et al. Preparation of LHRHa Paclitaxel liposomes and its anti tumor effect in vitro[J]. Journal of Chongqing Medical University, 2012,37(4):298-301. DOI：

摘要

目的：制备促黄体激素释放激素类似物（Luteinizing hormone releasing hormone analogues

LHRHa)靶向紫杉醇脂质体（Paclitaxel liposomes

PTX-Lipo），研究其在体外增强紫杉醇（Paclitaxel

PTX）对卵巢癌A2780/DDP细胞的抑制作用。方法：采用薄膜超声法制备PTX-Lipo与LHRHa靶向紫杉醇脂质体（LHRHa-Paclitaxel liposomes

LHRHa-PTX-Lipo），用透射电镜考察脂质体形态；高效液相色谱法测定2种PTX-Lipo的包封率；激光共聚焦法通过卵巢癌A2780/DDP细胞对4-氟-7-硝基-2，1，3-苯并氧杂恶二唑荧光素的摄取检测来反映细胞对NBD-Lipo与NBD-LHRHa-Lipo的摄取情况；MTT法及细胞克隆形成实验检测LHRHa-PTX-Lipo体外对卵巢癌细胞的生长抑制情况。-结果：制备LHRHa-PTX-Lipo的平均粒径123.4 nm，包封率在90%以上;A2780/DDP细胞对NBD-LHRHa-Lipo组的荧光摄取明显高于NBD-Lipo组；LHRHa-PTX-Lipo对A2780/DDP细胞的生长及克隆形成抑制明显高于PTX组及PTX-Lipo组（P＜0.05）。结论：采用薄膜超声法制备的LHRHa-PTX-Lipo可使药物在靶部位聚集，增强药物对卵巢癌细胞的抑制作用。

Abstract

Objective:To prepare LHRHa-Paclitaxel liposomes and to observe its inhibitory effect on ovarian cancer A2780/DDP cells in vitro. Methods:Paclitaxel-liposomes(PTX-Lipo) and LHRHa-Paclitaxel liposomes (LHRHa-PTX-Lipo) were prepared by means of film ultrasound and their morphology was examined with transmission electron microscope.The encapsulation rates of PTX-Lipo and LHRHa-PTX-Lipo were quantified with high performance liquid chromatography (HPLC).The cellular intake of NBD-Lipo and LHRHa-NBD-Lipo was embodied through detecting the intake of NBD fluorescein by ovarian cancer A2780/DDP cells with laser confocal microscope.The inhibitory effect of LHRHa-PTX-Lipo on ovarian cancer cells in vitro was measured by MTT assay and colony forming assay.Results:The mean diameter of LHRHa-PTX-Lipo particles was 123.4 nm with encapsulation rate being above 90%.The fluorescein intake of A2780/DDP cells was higher in NBD-LHRHa-Lipo group compared with that in NBD-Lipo group.The inhibitory effect of LHRHa-PTX-Lipo on the growth and clone formation of A2780/DDP cell was much more intense than that of PTX and PTX-Lipo(P<0.05).Conclusion:LHRHa-PTX-Lipo prepared by means of film ultrasound could aggregate drugs at targeted site thus increase the inhibitory effect of drugs on ovarian cancer cells in vitro.

关键词

脂质体紫杉醇靶向递送促黄体激素释放激素类似物生物素亲和素系统卵巢癌

Keywords

liposomepaclitaxeltargeted deliveryLuteinizing hormone releasing hormone analogues(LHRHa)biotin avidin systemovarian cancer

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