Objective：To study the expression of Galectin-3，Wnt pathway related protein（β-catenin，Cyclin D1 and E-cadherin） in salivary gland tumors and to explore the relationship and the clinical significance among them. Methods：The benign and malignant tumors of salivary gland specimens were observed under HE staining and light microscopy. Immunohistochemical staining（S-P method） was applied to detect the expression and correlation of Galectin-3，β-catenin，E-cadherin and Cyclin D1 in 107 cases of salivary gland tumors（58 cases of malignant tumors and 49 cases of benign tumors） and 38 cases of chronic inflammation of salivary glands were enndled as negative control. Results：The positive rate of Galectin-3 was 72.4%（42/58） in malignant salivary tumor，higher than that in benign salivary tumor（P<0.05）. The positive rates of β-catenin and Cyclin D1 protein were higher in malignant salivary tumor[75.9%（44/58） and 81%（47/58）] than in benign salivary tumor（P<0.05）. There were significant positive correlations be－tween Galectin-3 and β-catenin（r=0.313，P<0.05）. Conclusions：Galectin-3，Wnt pathway related protein β-catenin and Cyclin D1 are correlated with each other，which may provide new sight in the treatment of salivary gland tumor.