Objective：To investigate the relationship between serum levels of leptin，fatty acid synthase（FAS），estrogen（E） and cardio－vascular calcification. Methods：Totally 187 patients were divided into two groups by coronary angiography：coronary heart disease group （n=98，53 males and 45 females） and non-coronary heart disease group（n=89，47 males and 42 females）. Coronary artery cal－cification scores were calculated respectively in two groups. Meanwhile，serum levels of leptin，FAS，E，triglyceride（TG），total cholesterol（TC），C-reactive protein（CRP），low density lipoprotein-cholesterol（LDL-C），high density lipoprotein-cholesterol（HDL-C），P3- and Ca2+ of the two groups were measured by radioimmunoassay. Results：Serum leptin and FAS concentrations as well as coronary artery calcification scores were significantly higher in coronary heart disease group than in non-coronary heart disease group（P<0.05）. But the serum level of E was lower in coronary heart disease group than in non-coronary heart disease group（P<0.05）. Level of cardiovascular calcification was positively correlated with leptin（r=0.640，P<0.05），FAS（r=0.420，P<0.05），TG（r=0.120，P<0.05），TC（r=0.179，P<0.05），CRP（r=0.125，P<0.05） but negatively with E（r=-0.453，P<0.05） and HDL-C（r=-0.110，P<0.05）. Stepwise multiple regression analysis showed that factors of leptin，FAS，E，TG，TC and HDL-C entered the regression equation. Conclusions：Serum level of E is lower in those with coronary heart disease，which suggests that E may protect cardiovascular by antagonizing vas－cular calcification. Serum leptin and FAS concentrations of those with coronary heart disease are significantly increased indicating that leptin and FAS may promote the development of cardiovascular calcification.