Objective：To investigate the change of cortical blood flow and the dynamic changes of serum total cholesterol（TC） and high density lipoprotein（HDL） in brains of aging rat during cerebral ischemic injury. Methods：The model of aging rats with cerebral hypoperfusion was successfully constructed by persistent bilateral common carotid artery ligation（2VO）. The cortical blood flow and the concentration of serum TC and HDL at different time points were determined and compared. Results：Compared with the sham-operated group，the temporal blood flow was significantly decreased in 14 d group. But the collateral vessels were gradually regenerated and formed in local brain，while the temporal blood flow was restored in 21 d and 28 d group. The concentration of HDL and TC was significantly higher in 14 d group than in the sham-operated group（P<0.05），and both of them were decreased with the extention of ischemia time. Conclusions：The serum TC and HDL concentration undergo dynamic changes-increasing first and then decreasing during the process of cerebral hypoperfusion，which suggests that aging rats of cerebral hypoperfusion have unbalanced metabolism and display stress regulatory phenomenon due to cerebral hypoperfusion.