Objective：To investigate the effect of claudin-1 on the cell adhesion，invasion and metastasis capacity through inhibiting the expression of claudin-1 in SW620 cells. Methods：The expression of claudin-1 in SW620 cell was inhibited through shRNA in－terfering technique to detect its effect on cell adhesion ability. Then Transwell chamber invasion assay and cell scratch test were applied to detect the invasion and metastasis of cells. Results：Low expression of claudin-1 can inhibit adhesion of SW620 cells to laminin，fibronectin，collagen Ⅳand CollagenⅠ，with adhersive inhibition rate of 61%，29%，48%，12% respectively. The cell invasiveness was stronger in SW620-control group than in SW620-shRNA group，with inhibition rate of 70%（P<0.001）. After the inhibition of claudin-1，cell migration was significantly reduced. Conclusion：Inhibiting claudin-1 expression could suppress the adhesive and invasive a－bility of SW620 cells.