Objective：To screen and prepare the new carrier for Paclitaxel-loaded nanoparticles from bovine serum albumin by prote－olysis and nanoparticle technology and to study the physical and chemical indicators. Methods：Bovine serum albumin（BSA） which was degraded to fragments by pepsin was used as a carrier，the Paclitaxel-BSA fragments-nanoparticles were prepared by homoge－nized-spin steam. The appearance，size distribution，yield，encapsulation efficiency，drug loading and release in vitro of the sample were assessed. Results：Four clip groups were got through column chromatography after BSA enzymolysis. The fourth group was cho－sen as the candidate carrier. The nanoparticles got were round and uniform in shape with an average diameter of 150.2 nm. The aver－age yield of nanoparticles was（45.132±0.903）%，average encapsulation efficiency was （50.246±0.712）%，average drug-loading rate was （4.804±0.101）% and the 48 h accumulative release percentage was 82.4%. Conclusions：The preparation of Paclitaxel-loaded nanoparticles through BSA enzymolysis is feasible. Therefore，there is possibility to increase the sources of carriers for Pacli－taxel-loaded nanoparticles and to reduce the cost of prepara－tion.