Objective：To explore the influence of newcastle disease virus（NDV） replication in LX-2 cells on the viability and biologi－cal function of hepatic stellate cell（HSC）. Methods：Transforming growth factor-β1（TGF-β1）-stimulated LX-2 cells were infected by different titres of oncolytic NDV-Italien，the proliferation rate of LX-2 cells was detected by MTT assay. Immunofluorescent tech－nique was used to detect the expression of activation markers α-SMA in LX-2 cells. Results：NDV infection in LX-2 cells inhibited the cell proliferation in a dose-dependent manner. The growth inhibition rate of activated LX-2 cells was increased，the expression of activation markers α-SMA of LX-2 cells was down-regulated dramaticlly compared with those of controls. Conclusions：Replication of NDV in activated LX-2 cells inhibits the cell proliferation and attenuates the activation of LX-2 cells.