Objective：To investigate the reference intervals of serum Kalium ion（K+），Natrium ion（Na+），Chlorine ion（Cl-），total cal－cium（TCa），total carbon dioxide （TCO2），fasting blood glucose（FBG） and C-reactive protein（CRP） among healthy preschool children（aged from 3 to 6 years） by dry chemical system in Shenzhen. Methods：Stratified randomized cluster sampling method was used to select children aged from 3 to 6 years and 839 healthy children were selected for intravenous blood extraction. Serum K+，Na+，Cl-，TCa，TCO2，FBG and CRP in venous blood were detected by the VITROS 350 system and the data were analyzed by statistical meth－ods. Results：Reference intervals of serum K+，Na+，Cl-，TCa，TCO2，FBG and CRP were respectively 3.8-4.9，136-146，102-110，2.29-2.63，20-28，4.0-6.1 mmol/L and 0-7 mg/L. Conclusions：Reference intervals of serum K+，Na+，Cl-，TCa，TCO2，FBG and CRP are established by dry chemical system among healthy preschool children in Shenzhen. It can provide better references for clinical di－agnosis and treatment.