Objective：To discuss the feasibility of easy identification of cerebral spinal fluid leak with micro sample through self con－trol study. Methods：Totally 44 cerebral spinal fluid samples were collected. Olympus au2700 automatic biochemical analyzer and Johnson onetouch ultral blood glucose monitoring system were used to detect their glucose content respectively and to analyze their relationship. Results：There was a positive linear correlation between the outcomes of Olympus au2700 automatic biochemical analyzer and Johnson onetouch ultral glucose monitoring system（r=0.97，P<0.05）. Whereas the mean value of the Johnson onetouch ultral group was significantly higher than that of Olympus au2700 group（t=14.14，P<0.05）. After a proper calibration of linear regression square（GJP=0.65GJ+0.21），there was no significant statistical discrepancy between the two groups（t=0.01，P >0.05）. The average error of using Johnson onetouch ultral blood glucose monitoring system to detect the glucose content of cerebral spinal fluid was 0.12 mmol/L，with an accuracy of 95.7%. Conclusions：Johnson onetouch ultral blood glucose monitoring system，when used properly and followed with correct adjustment，can detect the glucose content of cerebral spinal fluid accurately in less than five seconds with just 0.05 ml sample，making the qualitative diagnosis of cerebral spinal fluid fast and easy and providing a new method in the diagnosis of cerebral spinal fluid leakage.