Objective：To investigate the effects of cold preservation reperfusion injury（CPRI） on the variation of bile acid composition in rats after liver transplantation. Methods：Reverse phase-high performance liquid chromatography with precolumn derivatization and fluorescence detection was used to determinate the bile acid composition in rat bile after the surgery by using the donor livers which were coldly preserved for 12 h，1 h and the control livers. Metabolite profiling analysis of bile acid was performed by the methods of principal component analysis and partial least squares-discriminant analysis. Results：It was obvious that CPRI for 12 h，1 h groups and the control group can be separated with the metabolite profile of bile acids and bile duct injury was probably closely related with taurodeoxycholic acid. Hydrophobicity index of CPRI for 12 h group was increased significantly after liver transplantation and was closely correlated with cold preservation time of the donor livers. Conclusion：Metabolite profiling of bile acids in rat bile af－ter liver transplantation is significantly influenced by CPRI.