Objective：To investigate the infection condition and distribution regularity of human papilloma viruses（HPV） in Yunnan province and to provide references for epidemiologic studies of HPV in this district. Methods：Totally 5 376 female subjects were de－tected using flow-through hybridization and gene chip technique for HPV genotyping. Infection rate of HPV and the proportion of different HPV subtypes in Yunnan province were counted. Results：（1）Total infection rate of HPV was 12.8% with 20 out of 21 HPV genotypes being detected. Infection rate of single genotype was 10.4%，of two genotypes was 1.8% and of three or more than three genotypes was 0.6%. （2）The most common HPV genotypes were HPV52 and HPV16，followed by HPV58，53，33 and HPV18. Infec－tion of all of these 6 HPV genotypes accounted for 65.4% of the total infection. （3）Infection rates of high risk HPV genotypes in the age groups of ≤25，26-35，36-45 and ≥46 were 7.4%，13.2%，11.6% and 10.3% respectively，with statistical differences between groups（ χ2=15.33，P <0.01）. Infection rates of low risk HPV genotypes in the age groups mentioned above were 1.2%，2.5%，2.2% and 1.3% respectively，without statistical differences between groups（ χ2=6.44，P >0.05）. Conclusions：HPV infection rate in Yunnan province is lower than the average level of China with single genotype infection being the most common. HPV52 and HPV16 are the high risk HPV genotypes and CP8304，HPV11，HPV6 are the low risk HPV genotypes. Infection rate of each HPV genotype is as－sociated with age. Results obtained in this study can provide thoretical evidence for the prevention and treatment of cervical cancer.