Objective：To explore the pathological changes of acute ethylene glycol（EG） poisoning and to provide the objective diagno－sis index for its forensic identification. Methods：Totally 30 SD rats were randomly divided into control group and acute EG poisoning group. Animals in acute EG poisoning group were poisoned with EG（12 ml/kg） in a manner of gastric perfusion. Tissue samples of heart，liver，and kidneys in each group were obtained and HE staining was conducted. Besides，transmission electron microscopy（TEM） was also used to observe the tissue samples from kidney and liver of each group. Results：In acute EG poisoning group，all tissue sam－ples showed different degrees of pathological damage，especially in kidney，in which the light-blue calcium oxalate crystals were found.Under TEM，circular or oval-shaped high density electronic particles depositions（calcium oxalate crystals） were observed in re－nal tubular epithelial cells as well as in liver cells. Conclusions：Calcium oxalate crystals deposite in various organs and pathological damages mainly locate in kidney which may provide the objective diagnosis index for forensic identification of acute EG poisoning.