Objective：To investigate the effects of the gentle liver manipulation，including toching，retracting and moving liver lobes gently，on hepatic ischemia-reperfusion injury during hepatic ischemia and its mechanism. Methods：Thirty SD rats were divided into three groups randomly：sham-operated group（sham group），ischemia reperfusion group（IR group：produced by total inflow occlusion for 15 min） and manipulation group（MN group：induced with 13 min gentle manipulation including touching，retracting and moving lobes during 15 min ischemia）. Serum aminotransferases alanine transaminase and aspartate aminotransferase（ALT and AST），tumor necrosis factor-α（TNF-α） and Pselection in the liver were measured after reperfusion. Results：In MN and IR groups，serum ALT and AST（P=0.000） as well as pathological injury scores（P=0.000） were increased；TNF-α and P-selection expressions were higher（P=0.000） compared with those in sham group. Those variables mentioned above were significantly increased in MN group compared with those in IR group（P=0.000）. Conclusions：Gentle in vivo liver manipulation increases the reperfusion injury during hepatic is－chemia，which may be caused by the decrease of pro-inflammatory and activity of neutophils.