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广州军区广州总医院妇产科,广州,510010
纸质出版日期:2014,
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周 雯, 郝辰珺, 关 婷. Has-miRNA-96在宫颈癌组织中的表达及其临床意义[J]. 重庆医科大学学报, 2014,38(3):405-408.
Zhou Wen, Hao Chenjun, Guan Ting. Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer[J]. Journal of Chongqing Medical University, 2014,38(3):405-408.
目的:探讨Has-miRNA-96(miR-96)在宫颈癌组织中的表达情况及其与临床病理特征间的关系。方法:采用茎环real-time PCR方法检测52例宫颈癌、28例正常宫颈组织中miR-96的表达,分析miR-96表达与宫颈癌常见临床病理特征的关系。结果:miR-96在宫颈癌组织中的相对表达量(127.045±235.424)高于正常宫颈组织(0.995±0.236),差异有统计学意义(P=0.000);在中、低分化癌的相对表达量(137.778±249.981)高于高分化癌中的相对表达量(75.766±147.237),差异有统计学意义(P=0.005);在腺癌中相对表达量为(196.213±172.519),明显高于鳞癌(110.576±246.910)(P=0.004);临床Ⅰ期、Ⅱ期、Ⅲ~Ⅳ期子宫颈癌患者组织中miR-96相对表达量分别为(22.614±25.828)、(122.493±78.043)、(672.902±476.169),Ⅲ~Ⅳ期高于Ⅰ期、Ⅱ期(P=0.000、P=0.001),Ⅱ期高于Ⅰ期,差异有统计学意义(P=0.000);有淋巴结转移组相对表达量为(142.537±104.673)明显高于无淋巴结转移组(19.787±26.204,P=0.000);浸润深度:≥1/2间质相对表达量为(75.323±94.822),高于<1/2间质者(27.449±49.728,P=0.025);而与患者年龄无明显关系(P=0.385)。结论:miR-96在宫颈癌组织中存在异常高表达,提示其可能在宫颈癌的发生发展中发挥致癌基因的作用,并且与宫颈癌的演进及不良预后密切相关。
Objective:To evaluate has-miRNA-96(miR-96) expression in cervical cancer tissues and their association with the clini-copathological features. Methods:Expression of miR-96 in 52 cases of cervical cancer tissues and 28 cases of normal tissues was examined by stem-loop real-time PCR. Correlations between expression of miR-96 and related clinicopathologic features of cervical cancer(age,pathologic pattern,histological grade,depths of interstitial infiltration,lymph node metastasis,clinical stage) were further analyzed. Results:Relative expression of miR-96 was significantly higher in cancer tissues(127.045±235.424) than in normal tis-sues(0.995±0.236)(P=0.000). Expression of miR-96 was significantly higher in middle and low differentiated carcinoma(137.778±249.981) than in high differentiated carcinoma(75.766±147.237),with statistical differences(P=0.005). Expression of miR-96 was significantly higher in cervical adenocarcinoma(196.213±172.519) than in squamous cell carcinomas(110.576±246.910)(P=0.004). Expression of miR-96 at Ⅰsatge,Ⅱstage and Ⅲ-Ⅳ stage was (22.614±25.828),(122.493±78.043) and (672.902±476.169) respectively;expression at Ⅲ-Ⅳ stage was significantly higher than that at Ⅰstage and Ⅱstage(P=0.000,P=0.001) and expression at Ⅱstage was significantly higher than that at Ⅰstage(P=0.000). Expression of miR-96 was significantly higher in those with lymphatic metastasis(142.537±104.673) than those without(19.787±26.204)(P=0.000). Patients with invasive depth<1/2 in-terstitial substance(27.449±49.728) showed lower level of miR-96 than those with invasive depth ≥1/2 interstitial substance(75.323±94.822)(P=0.025). Expression of miR-96 was not correlated with age(P=0.385). Conclusions:Aberrant expression of miR-96 sug-gests it might play a role as an oncogene in the tumorigenesis and development of cervical carcinoma and it possibly correlates with progression and prognosis of cervical cancer.
Has-miRNA-96(miR-96)宫颈癌茎环real-time PCR临床意义
has-miRNA-96(miR-96)cervical cancerstem-loop real-time PCRclinical significance
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