Objective：To explore the specific value of transvaginal B-ultrasound（TVS） in monitoring endometrium of infertility patients. Methods：Totally 500 infertility patients were randomly selected and analyzed retrospectivelyy. Endometrial thickness and morphology during the ovulation period was monitored by TVS. Results of TVS were divided into no obvious abnormality，endometrium thickness≤ 8 mm，≥16 mm and uneven endometrial echo four categories and hysteroscopy examination was combined. Results：There was no difference between TVS and hysteroscopy examination in detecting endometrial lesions（ ?字2=4.824，P=0.185）. Conclusions：TVS screening is one of the best and reliable and noninvasive auxiliary examination methods in detecting endometrial lesions of infertility patients，which can effectively improve the clinical diagnostic rate.