Objective：To detect the expression of VE-cadherin in different non-small cell lung cancer cell（NSCLC） lines with distinct invasion performance and to investigate the effects of the VE-cadherin silence on the invasion and metastasis of NSCLC cell lines. Methods：Expression of the VE-cadherin mRNA and protein were detected by quantitative PCR and Western blot in NSCLC cell lines with highly invasive，normally and low invasive ability（PG，H1299 and PA）. Effects of expression of VE-cadherin on the invasion and metastasis of PG cell line were explored by RNA interference. Results：VE-cadherin expressed in 3 kinds of NSCLC cell lines.mRNA and protein expression intensity of VE-cadherin was higher in PG，followed by H1299 and PA cell lines with statistical differences between PG and H1299（P=0.000） and statistical differences between PA and H1299，PG（P=0.000）. Result of Transwell chamber matrigal invasion assays revealed that the numbers of invasive cells of control group and interference group were 34.33 ±3.06 and 86.00±7.93 respectively，with statistical differences between groups（P=0.000）. Results of scratch damage experiment showed that the migration abilities between control group and interference group had no obvious differences in the first 24 h（P=0.825）. But after 48 h or 72 h，metastasis distance of control group was farther than that of interference group with statistical differences（P=0.000）. Invasion and metastasis of PG cell were down regulated by the reduction of the expression of VE-cadherin. Conclusions：VE-cadherin may play a critical role in the invasion and metastasis of NSCLC and may become a new target in the prevention and treatment of invasion，angiogenesis and metastasis of NSCLC.