Objective：To develop an analytical method based on high performance liquid chromatography（HPLC） for the determination of azodicarbonamide（ADC） in flour. Methods：Samples were extracted with acetone，degreased with n-hexane，then separated with a agilent Eclipse XDB-CN column（250 mm× 4.6 mm，5 μm） using acetonitrile-ammonium acetate（20 mmol/L） as mobile phase with a gradient elution program and were detected with diode array detector with the wavelength of 245 nm. Results：ADC had a good linear relationship within the range of 1.0-90.0 mg/kg；the correlation coefficient was higher than 0.99 and the limit of quantification（S/N≥10） was 5.0 mg/kg. Average recoveries of ADC at spiking levels of 5.0，10.0，45.0 mg/kg were ranged from 68.6% to 116.9% and relative standard deviations were less than 7.0%. Conclusions：The method developed is simple，rapid，accurate and reliable；it can be used for the determination of ADC in flour.