Objective：To compare the endogenous metabolites in the plasma of people with qi-deficiency constitution after the inter－vention by Buzhongyiqi pills using nuclear magnetic resonance（NMR）-based metabonomics and to find the possible specific metabo－lites. Methods：Twenty healthy people with qi-deficiency constitution were recruited and divided into control group and intervention group. Buzhongyiqi pills were used to do the intervention and the plasma was tested by NMR-based metabonomics before and after the intervention. Multivariate statistical analysis was done to find the serum-derived metabolic differences after the intervention by Buzhongyiqi pills. Results：The plasma before and after the intervention was analyzed by Simca-P and 45 changed chemical shift val－ues（VIP>1，P<0.05） were selected. Thirty specific metabolites were found through human metabolite database. Conclusion：These differences in metabolites are related to the tricarboxylic acid cycle. Buzhongyiqi pills can improve the disorder of energy metabolism，memory deterioration and fatigue of qi-deficiency population，which may associated with its regulation of GABA，phosphatidyl－choline，blood ammonia concentration.