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1. 贵阳医学院法医学系, 5,贵阳,50004
2. 2. 重庆医科大学基础医学院法医学教研室,重庆,400016
纸质出版日期:2015,
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王 尧, 杜琳敏, 袁增琼, 等. 铜绿蝇幼虫在不同脏器组织源食物上生长发育变化[J]. 重庆医科大学学报, 2015,40(2):285-288.
Wang Yao, Du Linmin, Yuan Zengqiong, et al. Growth and development of lucilia cuprina larvae fed by different organ tissues[J]. Journal of Chongqing Medical University, 2015,40(2):285-288.
目的:研究铜绿蝇幼虫在不同组织源的食物上生长发育变化。方法:在26 ℃恒温、相对湿度70%条件下,用猪的肌肉组织、肝脏组织、脑组织、肌肉与脂肪的混合物(6︰4)分别饲养铜绿蝇幼虫。每隔12 h测体长和体质量,化蛹后测蛹的体长和体质量。统计幼虫及蛹的死亡率。推算各组幼虫发育总历期和成虫的性别比。结果:40%脂肪混合组与其他3组相比生长缓慢,达到最大个体的时间延迟24 h以上。肝脏组幼虫体长(15.02±1.39) mm和40%脂肪混合组体长(14.85±1.43) mm明显小于脑组织组(17.53±2.08) mm、肌肉组(16.82±2.15) mm(F=5.440,P=0.003);肝脏组幼虫体质量(72.8±14.2) mg和40%脂肪混合组体质量(70.4±15.4) mg均明显小于脑组织组(89.6±19.2) mg和肌肉组(85.9 ±16.7) mg(F=3.320,P=0.030)。40%脂肪混合组蛹体长及体重均明显小于脑组织组和肌肉组(F=7.890,P=0.001;F=9.280,P=0.001)。40%脂肪混合组幼虫和蛹的死亡率均明显高于脑组织组、肌肉组、肝脏组(F=3.470,P=0.040;F=4.720,P=0.035)。结论:铜绿蝇幼虫在40%脂肪混合食物上生长发育历期较长,肝脏组和40%脂肪混合组幼虫及蛹的体长和体质量较小。
Objective:To research the growth and development of lucilia cuprina larvae fed by different organization sources. Methods:Under the condition of constant temperature(26 ℃)and relative humidity(70%),muscle tissue,hepatic tissue,brain tissue,muscle and fat mixture(6∶4) were used to feed lucilia cuprina larvae. Length and weight of larvae and were measured at an interval of 12 h while length and weight of pupa were measured after eclosion. Development stages of larvae,mortality of larvae and pupa as well as sex ratio of adults were recorded. Results:Growth was retarded in 40% fat mixed group than in the other three groups,achieving maximum individual delay time of 24 h. Larvae body length of hepatic tissue group(15.02±1.39) mm and 40% fat mixed group(14.85±1.43) mm were statistically shorter than those of brain tissue group(17.53±2.08) mm and muscle tissue group(16.82±2.15) mm(F=5.440,P=0.003). Larvae body weight of hepatic tissue group(72.8±14.2) mg and 40% fat mixed group(70.4±15.4) mg were statistically lighter than those of brain tissue group(89.6±19.2) mg and muscle tissue group(85.9±16.7) mg(F=3.320,P=0.030). Pupa body weight and body length of 40% fat mixed group were statistically lighter and shorter than those of brain tissue group and muscle tissue group(F=7.890,P=0.001;F=9.280,P=0.001). Larvae and pupa mortality of 40% fat mixed group were statistically higher than that in the other three groups(F=3.470,P=0.040;F=4.720,P=0.035). Conclusion:Lucilia cuprina larvae fed by 40% fat mixed food have longer growth and development period. Body weight and body length of larvae and pupa are lighter and shorter in hepatic tissue group and 40% fat mixed group than in the other groups.
死后间隔时间铜绿蝇幼虫法医昆虫学
postmorterm intervallucilia cuprina larvaeforensic entomology
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