Objective：To detect the different expressions of GM130，14-3-3ζ and integrin α3 in normal gastric tissues，moderately differentiated gastric cancer tissues and poorly differentiated gastric cancer tissues thus to explore its relationship with gastric cancer. Methods：The expression of ＧＭ130，14-3-3ζ and integrin α3 in gastric carcinoma tissues of 84 patients with gastric cancer and the 84 samples of normal gastric tissues（from 5 cm above the edge of the gastric tumor tissues） were detected by SABC immunohisto-chemistry. The expression of proteins and the clinical pathological data were analyzed. The expression of mRNA and protein of ＧＭ130，14-3-3ζ and integrin α3 were detected by RT-PCR and Western blot. Results：The positive expression rates of ＧＭ1301，14-3-3ζ2 and integrin α33（88.1%，90.5%，95.2%） were significantly higher in gastric cancer tissues than those in normal gastric tissues（52.4%，27.4%，42.9%）；the expression of the above three indicators in poorly differentiated gastric cancer group were higher than those in moderately differentiated gastric cancer group，with statistically significant differences（P1=0.000，P2=0.007，P3=0.000）. Spearman correlation analysis showed that positive correlation were observed in ＧＭ130，14-3-3ζ and integrin α3 in gastric cancer（P＜0.05）. The expression of ＧＭ130，14-3-3ζ and integrin α3 in gastric cancer was obviously related with pathological differentiation and clinical stage（P<0.05），but its correlation with age，gender，tumor location，tumor size infiltration depth or whether the lymph node metastasis was not found（P ＞0.05）. The levels of mRNA and proteins were higher in gastric cancer group than in normal gastric tissue group，higher in poorly differentiated gastric cancer than in moderately differentiated gastric cancer，with statistically significant differences between the two groups（P＜0.05）. Conclusion：The abnormal expression of GM130 may play an important role in the occurrence and development of gastric cancer.