HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性

楼 梦; 贾 英

您当前的位置：

首页 >

文章列表页 >

HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性

心血管疾病血液疾病2024神经精神疾病的治疗 | 更新时间：2023-12-18

- HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性
- Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship
- 重庆医科大学学报 2015年40卷第4期页码：604-606
- 作者机构：
  
  重庆医科大学附属第一医院妇产科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2015，
扫描看全文
楼梦, 贾英. HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性[J]. 重庆医科大学学报, 2015,40(4):604-606.

Lou Meng, Jia Ying. Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship[J]. Journal of Chongqing Medical University, 2015,40(4):604-606.
楼梦, 贾英. HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性[J]. 重庆医科大学学报, 2015,40(4):604-606. DOI：

Lou Meng, Jia Ying. Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship[J]. Journal of Chongqing Medical University, 2015,40(4):604-606. DOI：

摘要

目的：探讨宫颈癌组织中缺氧诱导因子-1α（hypoxia induced factor-1α）和MicroRNA 210（miR-210）之间的关系，为宫颈癌的早期发现寻找参考指标。方法：采用免疫组化En Vision法和real-time PCR分别检测52例宫颈鳞癌及20例正常宫颈上皮组织中 HIF-1α和 miR-210的表达情况，用Spearman秩相关检验分析癌组织中HIF-1α和miR-210之间的相关性。结果：宫颈鳞癌组织细胞大部分胞核表达HIF-1α，少部分胞核和胞浆同时表达，HIF-1α在宫颈鳞癌组织中的表达率为73.1%（38/52），正常宫颈组织细胞中无表达。miR-210在宫颈癌组织中的表达水平较正常宫颈组织显著上调，正常宫颈组织miR-210的表达水平为1.16±0.76，宫颈鳞癌组织中其表达水平为5.04±1.28（P=0.000）；在52例宫颈癌组织中，HIF-1α蛋白表达水平与miR-210表达呈正相关（r=0.43，P=0.001）。HIF-1α蛋白在正常宫颈组织中均无阳性表达，与miR-210无相关性。在所有72例样本中进行相关性分析，发现两者的表达呈正相关（r=0.67，P=0.000）。说明随着HIF-1α蛋白表达的增加，miR-210则随着增加。结论：HIF-1α和miR-210在宫颈鳞癌组织中均呈高表达，且两者有一定的相关性。推测HIF-1α和 miR-210与宫颈癌的发生发展相关，有助于进一步揭示宫颈癌的发病机制。

Abstract

Objective：To explore the correlation between hypoxia induced factor-1α（HIF-1α） and MicroRNA210（miR-210） in cer－vical cancer． Methods：The expression of HIF-1α protein was detected by immunohistochemistry. Poly（A）-RT-qPCR was used to de－tect the expression of miR-210 in cervical cancer and normal cervical tissues. The correlation between the expression of miR-210 and HIF-1α was analyzed in cervical cancer. Results：The expression of HIF-1α in cervical cancer was 73.1% （38/52），but HIF-1α was not detected in normal cervical tissues；the expression of miR-210 was significantly increased in cervical cancer tissues than in normal cervical tissues（P=0.000）. The expression of miR-210 in normal cervical tissues and cervical cancer was 1.16±0.76 and 5.04±1.28，respectively. The expression of HIF-1α and miR-210 were positively correlated in 52 cases of cervical cancer（r=0.43，P=0.001）. The expressions of HIF-1α and miR-210 were not correlated in normal cervical tissues. The expressions of HIF-1α and miR-210 were positively correlated in 72 cases（r=0.67，P=0.000）. Conclusion：The expression of HIF-1α and miR-210 are highly expressed in SCC tissues. HIF-1α and miR-210 are positively correlated in cervical cancer. It is predicted that HIF-1α and miR-210 are re－lated with the occurrence and development of cervical cancers，which is conductive to revealing the pathogenesis of cervical cancer.

关键词

宫颈癌缺氧诱导因子-1αmicroRNA210免疫组化real-time PCR

Keywords

cervical cancerhypoxia induced factor-1αmicroRNA210immunohistochemistryreal-time PCR

references

浏览量

253

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于图神经网络改进宫颈癌筛查系统与宫颈细胞DNA定量在高危型HPV阳性患者中的比较

人工智能技术在宫颈癌筛查和精准临床诊疗中的研究进展

CXCL10/CCL2 信号通路介导肿瘤微环境中宫颈癌的发展

流式免疫表型分析在11例侵袭性NK细胞白血病中的诊断价值分析

SMYD2在宫颈癌中的表达变化及意义