Objective：To construct pichia pastoris GS115 expression system of 15 kD recombinant human augmenter of liver regener－ation（rhALR），and to explore its effects on human hepatoma cell line QGY treated with cisplatin（DDP）. Methods：With polymerase chain reaction（PCR），and gene recombination techniques，cDNA of rhALR was obtained from recombinant plasmid pPIC9K-rhALR and inserted into plasmid pPICZαA. The recombinant plasmid pPICZαA-rhALR demonstrated by sequencing was linearized by di－gestion with SacⅠ，and transformed into GS115 with electroporation. The rhALR expression was secreted by GS115 under the induction of 10 mL/L methanol，and purified through Nickel column affinity chromatography after it was analyzed by Western blot（ALR poly－clonal antibody and His-tag mouse monoclonal antibody）. The effects of rhALR on in vitro proliferation of QGY and HepG2 cells，as well as its effects on reducing cellular proliferation inhibiton rate induced by DDP，were evaluated by MTS reagent. The anti-apoptosis effects of rhALR on QGY cells apoptosis induced by DDP were detected by flow cytometry. Results：The recombinant plasmid pPICZα－A-rhALR was identified by PCR，restriction enzyme reaction methods and direct sequencing respectively. RhALR as a secretory pro－tein was successfully expressed by GS115；with molecular weight about 17 kD，it accounts for 70% of the total protein in the super－natant from pPICZαA-rhALR GS115. The results of Western blot all showed the specific single band. The high qualitative rhALR pu－rified through Nickel column affinity chromatography could stimulate in vitro proliferation of human hepatoma cell lines（HepG2 and QGY） in a dose-dependent manner（F=246.729，P=0.000；F=246.004，P=0.000），reduce cellular proliferation in－hibition rate and play anti-apoptosis effect also in a dose-de－pendent manner when QGY cells treated with DDP（F=101.061，P=0.000）. Conclusion：The rhALR as a secretory protein is expressed in GS115 efficiently and purified through Nickel column affinity chromatography successfully. The rhALR exerts anti-apoptosis effects on QGY cells treated with DDP in a dose-dependent manner in vitro.