Objective：To investigate the influencing factors of differentiation degree of early gastric cancer（EGC） and to provide theo－retical basis clinically. Methods：The clinical data of gastric cancer patients with D2 lymph node dissection who underwent surgery in the First Affiliated Hospital of Chongqing Medical University from January 2009 to January 2014 were collected. The clinical data of 97 early gastric cancer patients were retrospectively analyzed. Tumor size，general type，tumor location，depth of invasion，lymph node metastasis，tumor markers，blood line transfer，gender，age and differentiation degree were analyzed using single factor and multiple factors analysis. Results：Logistic regression analysis showed that the undifferentiated carcinoma accounted for 73%（70/97）. Multiple analysis showed that female，<50 years old，1/3 part under the stomach were highly related to undiferentiated carcinoma（P<0.05）； OR，95%CI and P were（4.9，13.7，3.4），（2.4-13.6，4.2-24.1，1.7-8.4） and （0.019，0.013，0.027，respectively. However，there was no correlation between patients’ tumor size，general type，depth of invasion，lymph node metastasis，etc with undiferentiated carcinoma. Conclusion：The degree of differentiation in early gastric cancer has been proved to be mainly correlated with the gender，age，and tu－mor location.