Objective：To investigate the apparent diffusion coefficient（ADC） in dignosis of central gland prostate cancer and its corre－lation with Gleason score. Methods：The conventional MRI and DWI data of 20 cases of central gland prostate cancer and 20 cases of benign prostatic hyperplasia（BPH） were retrospectively analyzed. ADC between two groups were compared. ROC curves was used to evaluate the efficacy of ADC in diagnosing central gland prostate cancer. According to the results of Gleason score，the lesions were divided into 3 groups：low-risk group≤6 score，intermediate-risk group=7 score and high-risk group≥8 score. ADC between groups were compared and Spearman correlation analysis was used to evaluate the correlation between Gleason score and ADC. Results：There were statistically differences in ADC between central gland prostate cancer group and benign prostatic hyperplasia group（t=-4.361，P=0.000）. When the cutoff value was 1.158×10-3 mm2/s，the area under the ROC curve was 0.823. The diagnostic sensitivity，specificity was 80.8% and 80.0%. There were statistical differences in ADC among three groups in central gland prostate cancer（F=5.193，P=0.014）. There were statistical differences in ADC between low-risk group and intermediate-risk group，low-risk group and high-risk group（P=0.008，P=0.021）. There was no difference between the intermediate-risk and high-risk group in ADC（P=0.869），while there were statistical differences between low-risk and high-risk group in ADC（t=3.286，P=0.003）. Central gland prostate cancer showed negative correlation with Gleason score（r=-0.469，P=0.016）. Conclusion：ADC values have certain value in the diagnosis of prostate cancer in the central gland. ADC and central gland cancer Gleason score are negatively correlated. ADC may reflect the degree of differentiation of prostate cancer.