Objective：To investigate the association between serum CA19-9，CA125 levels and its related factors in T2DM patients. Methods：Totally 117 T2DM patients and 132 healthy subjects who had medical checkups were recruited to compare metabolic in－dices including blood glucose，lipids and protein as well as serum CA19-9 and CA125 levels. The change in CA19-9 and CA125 lev－els before and after treatment was observed. Results：（1）Statistically significant differences were found between normal controls and T2DM patients in serum CA19-9，CA125 levels and metabolic components including glucose，lipids and protein（all P＜0.05）. （2）There were statistical differences in serum glucose，CA19-9 and CA125 levels in T2DM patients with a HbA1c＞7.5% when compared with those with a HbA1c≤7.5%（all P＜0.05）. （3）When compared with patients with no complication and those with chronic compli－cations，T2DM patients with acute complications had statistically different serum levels of glucose，CA19-9 and CA125（all P＜0.05）. （4）Carbohydrate antigens of T2DM patients with abnormal CA19-9 or CA125 were significantly lower after treatment than before treatment（all P＜0.05）. （5）Correlation analysis revealed that glucose，HbA1c and total cholesterol were positively correlated with CA19-9，CA125 levels in T2DM patients. Serum albumin was negative correlated with CA19-9 and CA125 levels in T2DM patients. Conclusion：T2DM patients exists not only glucose and lipid metabolism disorders，but also disorders of protein metabolism. More evi－dent increase in CA19-9 and CA125 levels will arise if the T2DM patient has poorer glycemic control. With the improvement of glycemic control，CA19-9 and CA125 levels will decrease. The change in serum and CA125 levels in T2DM patients is likely to be associated with disorders of glucose，lipid and pro－tein metabolism.