Objective：To explore the detection results of DNA in human obsolete bone extracted by modified phenol-chloroform method and amplified with different kits. Methods：DNA in 30 femoral bones were extracted by modified phenol-chloroform method，fluorescent label and multiplex amplification with identifiler-plus kit，minifiler kit and godeneyeTM 20A kit，while the gene loci was detected and analyzed by genetic analyzer software. Results：The DNA gene loci in in human oboslete bone were 469，262，588 re－spectively，which were higher than those of 307，175，436 by traditional method. Conclusion：DNA in human obsolete bone might be extracted and detected effectively by modified phenol chloroform method and multiplex amplification with 3 kits.