Objective：To investigate the effect of colchicine on proliferation and cell cycle（G2）of human KFB. Methods：The primary human keloid-derived fibroblast were cultured by tissue. The proliferative behavior of KFB was studied after pre-treating with various concentrations of colchicines（1，2，4，8，16，32 μg/ml） by 4 methyl thiazolyl tetrazolium method and the cell cycle was assessed by flow cytometry. Results：MTT analysis showed that 1 μg/ml group（12.56±0.79）%，2 μg/ml group（27.35±0.50）%，4 μg/ml group（34.36±0.25）%，8 μg/ml group（39.38±0.57）%，16 μg/ml group（40.38±0.23）%，32 μg/ml group（44.65±0.60）%，F=2440.178，P=0.000；flow cytometry analysis showed that blank control group（0.67±0.46）%，1 μg/ml group（4.26±1.51）%，2 μg/ml group（24.51±4.69）%，4 μg/ml group（45.12±2.75）%，8 μg/ml group（55.81±5.04）%，16 μg/ml group（69.90±3.61）%，32 μg/ml group（85.53±2.06）%，F=491.609，P=0.000. Not only was the proliferation of KFB was inhibited，but also the prophase of cell division（G2） was af－fected depending on the time and drug concentration. Conclusion：The primary human keloid-derived fibroblast should be cultured by tissue adherent culture method and colchicine may regulate the proliferation and prophase of cell division of KFB at 1-32 μg/ml in 24 hour.