Objective：To investigate hepatitis C virus（HCV） genotypes distribution，evolution and possible clinical significance in Chongqing area，and to provide basic evidence for better treatment and prevention of HCV. Methods：HCV genotype distribution of hepatitis C patients who had information of HCV genotypes from January 2010 to December 2013 diagnosed in the Second Affiliated Hospital of Chongqing Medical University were retro－spectively analyzed and compared with the reported literatures in this area. The possible factors influencing evolution of HCV genotype were investigated. Results：Totally 941 cases were re－cruited in this study. Four genotypes and 9 genetic subtypes of HCV were founded among the 857 cases who had single genotype（the typing rate was 99.1%），and the genotypes were not clearly typed in the other 84 cases. Among those genotypes and genetic subtypes，there were 30 cases（3.5%）genotype 1a，238 cases（27.8%） 1b，42 cases（4.9%） 2a，1 cases（0.1%）2b，132 cases（15.4%）3a，244 cases（28.5%）3b，6 cases（0.7%）3k，124 cases（14.5%） 6a and 40 cases（4.7%） 6b. Compared with the results of 5 years ago，1b and 2a genetic subtype were decreased obviously，and the proportion of genotype 3 and 6 increased. Conclusion：HCV genotype 3b and 1b are the predominant genotypes among HCV infection patients in Chongqing；however genotypes 3a and 6a also have relative high proportion，indicating the diversity and complexity of HCV genotype in Chongqing.