Objective：To explore the relationship between the expression of neuropilin-2（NRP2） and microRNA-486-5p（miR-486-5p） in gastric carcinoma（GC） and adjacent non-tumorous tissues and lymph node metastasis of GC. Methods：Sixty-five gastric carcinoma and adjacent non-tumorous specimens were collected. Immunohistochemistry（IHC） was used to detect NRP2 protein expression in tissues；quantitative real time PCR was used to detect the expression of miR-486-5p in tissues；microlymphatic density（MLD）was evaluated with lymphatic endothelium-specific marker-D2-40. The relationship between NRP2，miR-486-5p and MLD was analyzed. Results：Compared with those in normal gastric mucosa tissues，the expression of NRP2 was significantly up-regulated in GC tissues（P=0.000），while the expression of miR-486-5p was down-regulated significantly（P=0.000）. Statistic analysis revealed the expression of miR-486-5p in GC tissues was negatively correlated with MLD（P<0.001） and NRP2 was positive correlated with MLD（P=0.015）. Conclusion：The expression of NRP2 and miR-486-5p is closely associated with lymph node metastasis of gastric carcinoma，indicating that miR-486-5p and NRP2 might be used as targets for molecular therapy of gastric carcinoma.