Objective：To analyze the expression profile variation of long non-coding RNA（lncRNA） in 3-O-C12-HSL-inhibted Mo-DCs maturation. Methods：Human CD14+ monocytes were separated by immunomagnetic beads and induced by hIL-4 and hGM-CSF. Experimental group was stimulated with 40 μmol/L 3-O-C12-HSL and 0.1 μg/mL LPS；control group was stimulated with 0.1% DMSO and model group was stimulated with 0.1 μg/mL LPS. lncRNA chip was used to analyze the differential expression among control group，model group and experimental group. Significant differe-ntial lncRNA（P<0.05） was analyzed and scatter plot was used to study the variance in 2 times differential lncRNA. Clustering analysis was used to study the 5 times differential lncRNA. Results：Comparing with those of control group and model group，1 386 lncRNA which have more than 2 times variation and significant differences by statistical analysis were selected out，including 548 up-regulated lncRNA and 838 down-regulated lncRNA. Meatime，153 lncRNA accounting for 11.04％ of all differential lncRNA had more than 5 times variation in differ－ence groups. Characteristic change were displayed among 2 times differential lncRNA in scatter plot analysis. The hierarchical clustering results displayed that 5 times lncRNA clustering efficient in different groups. According to GO analysis and mRNA pathway analysis，PPAR signaling pathway，calcium signaling pathway and NF-κB signaling pathway were enriched with differential lncRNA associated genes. Conclusion：lncRNA displaying characteristic change may involve in 3-O-C12-HSL-inhibted Mo-DCs maturation.